Stage XY

Preparation

General requirements

General installation conditions: for a secure installation of FluidFM Bot positioning system on the superior unit (e.g. optical microscope) it must be ensured:

• Accessibility is provided
• Sufficient lighting is set up
• The unit is disconnected from the power supply and protected from unexpected switching on
• All adequate safety precautions are fulfilled
• Necessary measures for safety work are taken

Installation of the FluidFM XY

Unpacking of the FluidFM Bot positioning system

Open transport box and remove packaging.

FluidFM Bot XYZ positioning system in the transport crate

Lift the FluidFM XYZ positioning system from the crate

• Remove protective foam
• Open packaging foil with the scissors
• Remove packaging foil and put the positioning system on a clean, flat surface of the transport carriage
• Lift the Stage XYZ from the transport crate carefully

The positioning system weighs more than 25 kg.

Before lifting the system manually, the Fluid FM Bot Z-axis (8.5 kg) must be first disassembled from the FluidFM Bot XY-axis (16.5 kg) and be taken out separately.

FluidFM XY-stage

FluidFM Bot XY-stage transport safety (fitting screw)

Remove transport safety fitting screw, as showed in the video:

Installation of FluidFM Bot XY

Mounting position of the FluidFM Bot XY-stage in the superior unit (e.g. optical microscope) is horizontal.

Requirements for installation: interface for installation on the superior unit:

Stage XY is mounted with the screws onto the microscope. Mounting surface must maintain a flatness minimum 15 µm.

Depending on the microscope’s type must be used spacers or distance blocks. The assembly of FluidFM Bot XY-axis onto the Zeiss Observer and Olympus microscope is described below. The Nikon and Leica are not described in this manual.

It must be ensured that superior unit is disconnected from the main line during the XY-Stage installation. To ensure proper grounding of the FluidFM Bot XY-stage together with superior unit.

The support bolts stabilize the XYZ-System on the microscope.

Assembling and adjusting of the support bolts:

• Coarse setting of the height: install the support bolts and set their height by loosing of the clamp holders
• Precise setting of the height: screw down the bolts till they slightly place on the baseplate
• Counter the support bolts with ring nuts as showed in the video:

Zeiss Observer microscope

• No spacers or distance blocks are used by the mounting the XY-axis onto Zeiss microscope
• Set up carefully in twos XY-Stage onto the microscope
• Slide X-axis to left stop position. Milling grooves in X-sledge provides an access to the screw holes
• X-sledge must be placed in the appropriate position
• Mount and tighten the 3xM4x16 fixing screws (torque value 310 Ncm)
• Access to screws (crossed areas) can be possible in certain X-axis positions only

Interface for Zeiss Observer. Fastening with 3xM4x16 screws

(Vorderseite XY-Stage is Vorefront XY-Stage):

Olympus IX83 microscop

• Spacers or distance blocks are necessary for FluidFM Bot XY-axis mounting onto Olympus microscope
• 15 mm Parfocal length adapters have to be mounted below the objectives
• First fasten the distance blocks on the microscope
• Set up XY-Stage carefully in twos onto microscope
• Slide X-axis to left stop position. Special milling grooves in X-sledge provides an access to the screw holes
• Mount and tighten the 3xM4x12 fixing screws (torque value 310 Ncm). Access to screws (crossed areas) can be possible in certain X-axis positions only

View from below: distance blocks for Olympus microscope

View from above: tightening with 3xM4X12 screws