Bot Head

Deinstallation of the head

For safety reasons, a probe adaptor must be gripped on the FluidFM BOT.
Make sure that ARYA software is not running and that the BOT control unit is powered off.

From the back of the FluidFM BOT, open the incubator to access the head. Move the head to the highest position (z-axis).
Carefully, take out the pressure tube and the head cable out of the socket by pressing the clips on the sides.
With a 2mm Allen key, unscrew the 2 screws of the head, while keeping the head with one hand.
Carefully, pull up the head and remove it from the system.

Place the head on a flat and clean surface.
Using a T1 screwdriver, lock into the golden gripper and release the probe mounted on the head by rotating the screwdriver vertically down. Remove the probe manually.
Do the cleaning
- For a soft cleaning, clean the nose with isopropanol using lint-free cleaning sticks and wipes, without removing the sealing screw.
- For a thorough cleaning, after cleaning the nose as indicated before,
  - Remove the sealing screw: screw it out with a T1x40 screw driver and remove carefully the O-ring with tweezers.
  - Place it together with the O-ring into isopropanol.
  - Sonicate for 3-5 min until it is clean.
  - Wipe with lint-free tissue.
  - If necessary, replace the sealing screw and/or the O-ring for a new one.
  - Once it is dry, assemble the O-ring and the sealing screw: to drive it in correctly, first rotate it slightly anticlockwise and then start turning it clockwise. Tighten the sealing screw until dead stop (do not tighten the screw too much).
Mount the reference consumable.
Screw the head on the BOT and connect the microfluidics tubing and the control unit of the BOT.
Turn on the control unit, the FluidFM microfluidics system and ARYA.
Perform a XY and Z calibration of the head