Quick start guide
Before you start a new experiment, make sure that the required hardware is powered on and connected.
Check if these components are turned on and connected to the system
- FluidFM BOT controller
- FluidFM microfluidics controller
- Optical microscope (if motorized)
- Optical camera
- Barcode reader
Also, make sure your sample and your probe are ready.
After starting the program you are given a choice to continue the last experiment you were working on or to start a new experiment.
Projects and experiments are described in more detail here.
After starting or continuing an experiment, wait while the application initializes and configures connected hardware.
Error messages will be displayed at the bottom of the screen if errors occurred during the initialization process. Pressing the expansion button on the left side shows detailed error information.
Then insert the plates and configure the system by selecting the appropriate plate type. Press “To Left Port” and “To Right Port” to get access to the plate holder.
The software at a glance
- Interactive sample display
- The main section of the program shows a live video feed of the sample. Software elements such as the crosshair or a point preview are shown there depending on the context. The underlying sample can moved with normal dragging gestures.
- The toolbar provides access to standalone components.
- Depending on the selected tab settings or the list of available workflows are shown. Each workflow consists of several individual steps, where parameters and options can be chosen where necessary
- In tool, workflows and settings menus, parameters written in a bright, white font can be changed in an editor by simply touching or clicking them. In contrast, grey values are either in a blocked state or generally read-only and cannot be changed.
All your results are stored in a database on the FluidFM computer except for video files. Already acquired results can be inspected by expanding the media bar. The data can later be accessed and/or exported into standard formats, e.g. for presentation purposes or further processing. Export is available from ARYA but the specialized EDNA program provides more powerful browsing, viewing and exporting capabilities.